Part:BBa_K4612134

hsa-let-7a-2-5p _S2

hsa-let-7a-2-5p _S2(BBa_K4612134) is a single-stranded DNA sequence with 117 nucleotides that is a secondary weight probe for the target hsa-let-7a-2-5p. The sequence at its 3' end is designed to be complementary to the primary weight strand so that it can bind specifically to the primary weight strand of the primary weight probe when it is released, thus accomplishing recognition of the input signal. the DNA polymerase polymerizes DNA in the 5'-3' direction starting from the 3' end of the primary weight strand. In order to realize its target hsa-let-7a-2-5p weight number size 25, we adopt the cascade method to convert 25 into 5*5, so there are also 5 secondary weight strands bound to this probe, and the secondary weight strands will be replaced and released by newly synthesized sub-strands during the process of DNA polymerization by Bst DNA polymerase. Through this process, the signal of the target is quantitatively converted into the number of weighted strands, which facilitates subsequent calculations, and finally the results of the operation are converted into different fluorescence intensities, making the results simple and easy to read.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]