Part:BBa_K4612133
hsa-mir-148a-3p_S2
hsa-mir-148a-3p _S2(BBa_K4612133) is a 64-nucleotide single-stranded DNA sequence that serves as a secondary weight probe for the target hsa-mir-148a-3p. The sequence at its 3' end is designed to be complementary to the primary weight strand so that it can bind specifically to the primary weight strand on the primary weight probe when it is released, thus accomplishing recognition of the input signal.DNA polymerase polymerizes the DNA from the 3' end of the primary weight strand in a 5'-3' direction. In order to realize its target hsa-mir-148a-3p weight number size 10, we adopt the cascade method to convert 10 into 5*2, so there are also 2 secondary weight chains bound to this probe, and the secondary weight chains will be replaced and released by the newly synthesized sub-strands during the process of DNA polymerization by Bst DNA polymerase. Through this process, the signal of the target is quantitatively converted into the number of weighted strands, which facilitates subsequent calculations, and finally the results of the operation are converted into different fluorescence intensities, making the results simple and easy to read.
Sequence and Features
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