Part:BBa_K4612118
CD177_S
CD177_S (BBa_K4612118) is a single-stranded DNA sequence with 107 nucleotides and is a weighted probe for the target CD177. The sequence at its 3' end is designed to be complementary to the target CD177 so that it binds specifically to the target and thus accomplishes recognition of the input signal.DNA polymerase polymerizes the DNA in a 5'-3' direction starting at the 3' end of the target CD177.This The probe also incorporates a weighted strand corresponding to weight number size 4 of the target CD177, which is replaced and released by the newly synthesized daughter strand during DNA polymerization by Bst DNA polymerase. Through this process, the signal of the target is quantitatively converted into the number of weighted strands, which facilitates subsequent calculations, and finally the results of the operation are converted into different fluorescence intensities, making the results easy to read.
Sequence and Features
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