Coding

Part:BBa_K4609059

Designed by: Yisong Wen   Group: iGEM23_JLU-China   (2023-10-09)


lac operator

Lactose operon is a gene group involved in lactose breakdown, which is composed of repressors and manipulation sequences of the lactose system, allowing a set of genes related to lactose metabolism to be synchronously regulated. In the lactose system operon of E. coli, the structural genes of β-galactosidase, galactoside permease, and galactoside transacylase are arranged on the plasmid in the order of LacZ (z), Lac Y (y), Lac A (a), respectively. The operator sequence Lac O (o) is upstream of z, and the promoter Lac P (p) is further ahead. This is the structural pattern of the operon (lactose operon). The regulatory gene Lac I (i) encoding the repressor in the lactose manipulation system is located adjacent to and upstream of p.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None