Coding

Part:BBa_K4609053

Designed by: Yisong Wen   Group: iGEM23_JLU-China   (2023-10-09)


FRT-pBAD-HicA-FRT

A toxin that causes dormancy in E. coli. The natural HicA toxin cannot exist in the cell alone and requires the presence of the HicB antitoxin gene in the cell.FRT sites are added at both ends, which can bind to FLP proteins and be knocked out.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None