Part:BBa_K4597002
Periplasmic signal peptide (E.coli)
This signal peptide (PelB) and upstream translation initiation region (TIR) (contains Shine-Delgarno sequence) has been evolved to increase the expression of periplasmic proteins when bound to the N-terminal. The TIR includes the beginning of the signal peptide sequence as it was found that this part of the sequence was important for translation initiation, as well as the upstream untranslated region. Disulphide bonds do not generally form correctly in the cytosolic environment of Escherichia coli, so a signal peptide was required, which is able to efficiently get the protein transported to the periplasm where environmental conditions are more favourable to proper protein folding for the gastric intrinsic factor (GIF) protein. Mirzadeh et al. (2020) were able to evolve this PelB signal peptide to increase the periplasmic export of human growth hormone in E. coli, which is a different human protein but one that also required proper disulphide bond formation to function correctly.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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