DNA

Part:BBa_K4597001

Designed by: Dylan Brettingham   Group: iGEM23_Guelph   (2023-09-16)


E.coli GIF - optomized

The gene sequence for the human gastric intrinsic factor (GIF), a protein involved in vitamin B12 absorption, has had the codons optimized for expression in an Escherichia coli chassis. Vitamin B12 cannot be directly absorbed into intestinal parietal cells and must first be bound to GIF for transport into the cell. As GIF is normally produced by human cells, the unaltered gene sequence contains codons that are infrequently used in E. coli, affecting the synthesis of the protein, which is why we optimized the gene sequence for codons commonly used by E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/biosynthesis
Parameters
protein