Coding

Part:BBa_K4596003

Designed by: Junteng Sun   Group: iGEM23_OUC-Haide   (2023-08-20)


β-nicotinamide mononucleotide synthetase->FtNadE

This enzyme can catalyze the synthesis of nicotinamide mononucleotide (NMN), increase the content of NMN in vivo, and increase the precursor for the synthesis of non-natural coenzyme nicotinamide cytosine dinucleotide (NCD)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 154
  • 1000
    COMPATIBLE WITH RFC[1000]

Profile

Name: FtNadE
Base Pairs: 750 bp
Origin: Francisella tularensis, synthetic
Properties: Catalyze the synthesis of nicotinamide mononucleotide

References

Wang, X, Feng, Y, Guo, X, Wang, Q, Ning, S, Li, Q, Wang, J, Wang, L & Zhao, ZK 2021, ‘Creating enzymes and self-sufficient cells for biosynthesis of the non-natural cofactor nicotinamide cytosine dinucleotide’, Nature Communications, vol. 12, no. 1, p. 2116.

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