Part:BBa_K4594033

LuxB:B2

A fusion protein formed by linking the B2 fluorescent protein to the back of the LuxB fluorescent protein with a Glu-Leu linker. For more information about B2, please refer to BBa_K4594027 , and for LuxB, please refer to BBa_K3769013 . In iGEM 2023, ShanghaiTech_China expressed this fusion protein with LuxCDAE and LuxGF. The resulting spectrogram is presented below.

LuxB:B2 fluorescent protein after fluorescence resonance energy transfer the highest peak of the emission spectrum should be around 506nm, the experimental results show a double peak, the peak at 490nm is the highest peak of the emission light of the first protein. The experimental results are in line with the theoretical values.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1530
  Illegal AgeI site found at 1638
  
• 1000
  COMPATIBLE WITH RFC[1000]