Part:BBa_K4594030

LuxB:A1

A fusion protein formed by linking the A1 fluorescent protein to the back of the LuxB fluorescent protein with a Glu-Leu linker. For more information about A1, please refer to BBa_K4594007 , and for LuxB, please refer to BBa_K3769013 . In iGEM 2023, ShanghaiTech_China expressed this fusion protein with LuxCDAE and LuxGF. The resulting spectrogram is presented below.

LuxB:A1 fluorescent protein after BRET the highest peak of emission spectrum should be around 525nm. The experimental results show a double peak. The peak at 490nm is the highest peak of emission light of the first protein. The experimental results are in accordance with the theoretical values.

Attached below is a picture of the results.

 

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]