Composite

Part:BBa_K4594019

Designed by: Yueying Wang   Group: iGEM23_ShanghaiTech-China   (2023-10-04)


NSIII-2-PkaiBC-sfGFP with LVA-lacI-KanR-NSIII-1

The sfGFP in this part has a LVA degradation tag and is expressed under the control of PkaiBC. At the same time, it can be inserted into the genome of Synechococcus elongatus PCC7942 through NSIII. Therefore, this part can be used to verify the feasibility of the rhythmic expression of foreign genes in PCC7942.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 641
    Illegal PstI site found at 4497
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 641
    Illegal PstI site found at 4497
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 641
    Illegal PstI site found at 4497
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 641
    Illegal PstI site found at 4497
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None