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Part:BBa_K4594015

Designed by: Yueying Wang   Group: iGEM23_ShanghaiTech-China   (2023-10-04)


NSII

Neutral site 2(NSII) is another insertion site commonly used in transforming Synechococcus elongatus PCC7942. We plan to obtain this sequence from PCC7942 by PCR and use it for subsequent plasmid construction.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 152
    Illegal PstI site found at 830
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 152
    Illegal PstI site found at 830
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 152
    Illegal PstI site found at 830
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 152
    Illegal PstI site found at 830
  • 1000
    COMPATIBLE WITH RFC[1000]


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