Coding

Part:BBa_K4594012

Designed by: Shubai Wang   Group: iGEM23_ShanghaiTech-China   (2023-10-01)


PDHC


The pyruvate content in PCC7942 is very high, which is about 100 times the content of acetyl-CoA. Experiments have shown that the high expression of pyruvate dehydrogenase PDHC in PCC7942 can increase its carbon flux and thus improve its ability to produce foreign substances. Therefore, we designed a high expression of PDHC to increase the content of luminous raw materials in PCC7942.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 287
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 442
    Illegal PstI site found at 287
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 287
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 287
    Illegal NgoMIV site found at 183
    Illegal AgeI site found at 561
    Illegal AgeI site found at 1102
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None