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Part:BBa_K4594010

Designed by: Shubai Wang   Group: iGEM23_ShanghaiTech-China   (2023-10-01)


NSIII-2

Neutral site 3 (NSIII) is one of the insertion sites commonly used in the transformation of Synechococcus elongatus PCC7942. NSIII-2 acts as an upstream homology arm.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 641
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 641
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 641
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 641
  • 1000
    COMPATIBLE WITH RFC[1000]


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