Part:BBa_K4594002
luxF
luxF is a gene coding the protein LuxF, which is contained in some of phosphorous bacteria such as Photobacterium leiognathi subsp. mandapamensis. LuxF is a homodimeric protein forming a seven-stranded β/α fold.
During the structural characterization of the protein, a flavin derivative (myrFMN) was discovered that was tightly bound to the protein. It was reported that LuxF is important to prevent inhibition of the luciferase by myrFMN and thus it was proposed that LuxF acts as a scavenger of myrFMN. So, LuxF is an ideal part enhancing the brightness of the lux operon system. [1]
Fig 1 Structure of LuxF with myrFMN
Fig 2 Comparison of light intensities of E. coli-based lux operon expression system. A: Comparison of relative light intensities of Photobacterium mandapamensis 27561 with and without luxF gene. B: Comparison of relative light intensities of Photobacterium leiognathid 25521 with and without luxF gene.
[1] Brodl, Eveline et al. “The impact of LuxF on light intensity in bacterial bioluminescence.” Journal of photochemistry and photobiology. B, Biology vol. 207 (2020): 111881. doi:10.1016/j.jphotobiol.2020.111881
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