Part:BBa_K4590010

di-Tricarboxylate Transporter and Malonyl-CoA Synthase

Its contains both St(r)epF (BBa_K4590008) and St(r)epG (BBa_K4590009) together.

St(r)epF (BBa_K4590008) is a gene from Streptomyces hygroscopicus that encodes a protein similar to a di-tricarboxylate transporter in Rhizobium, and it is involved in malonate incorporation. St(r)epG (BBa_K4590009) is a gene from Rhizobium leguminosarum that encodes Malonyl-CoA Synthase and is involved in fatty acid synthesis and elongation.

Malonate serves as a precursor in the fatty acid pathway, which is necessary for anthracycline synthesis. Our goal is to increase Cosmomycin production by facilitating the incorporation of malonate into the microorganism and enhancing the flow of fatty acid synthesis (LIMA LOBATO, 2010).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2374
  Illegal PstI site found at 2032
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2374
  Illegal PstI site found at 2032
  Illegal NotI site found at 1072
  Illegal NotI site found at 1213
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2374
  Illegal BamHI site found at 461
  Illegal BamHI site found at 989
  Illegal XhoI site found at 1592
  Illegal XhoI site found at 2152
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2374
  Illegal PstI site found at 2032
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2374
  Illegal PstI site found at 2032
  Illegal NgoMIV site found at 52
  Illegal NgoMIV site found at 439
  Illegal NgoMIV site found at 1692
  Illegal NgoMIV site found at 1846
  Illegal NgoMIV site found at 1924
  Illegal AgeI site found at 2137
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 291
  Illegal SapI site found at 525
  Illegal SapI site found at 774