Coding
Str(e)pF

Part:BBa_K4590008

Designed by: Beatriz Lauriano de Oliveira   Group: iGEM23_USP-Brazil   (2023-10-02)


di-Tricarboxylate Transporter

St(r)epF (BBa_K4590008) is a gene from Streptomyces hygroscopicus that encodes a protein similar to a di-tricarboxylate transporter in Rhizobium. It is involved in the incorporation of malonate.

Malonate serves as a precursor in the fatty acid pathway, which is essential for anthracycline synthesis. Our goal is to enhance Cosmomycin production by facilitating the incorporation of malonate into the microorganism and increasing the flow of fatty acid synthesis (LIMA LOBATO, 2010).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1072
    Illegal NotI site found at 1213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 461
    Illegal BamHI site found at 989
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 439
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 291
    Illegal SapI site found at 525
    Illegal SapI site found at 774


[edit]
Categories
Parameters
None