Part:BBa_K4590007
ABC transporter and resistance genes
It contains St(r)epB (BBa_K4590002), St(r)epC (BBa_K4590003), St(r)epD (BBa_K4590005), and St(r)epE (BBa_K4590006) in a single vector.
When St(r)epB (BBa_K45900002) and St(r)epC (BBa_K4590003) are combined, they encode an ABC transporter, which will be utilized to export Cosmomycin into the extracellular environment (Castillo et. al 2022).
Membrane transporters play a crucial role in antibiotic resistance as they reduce the concentration of these compounds inside the cell. By overexpressing the ABC transporter, our goal is to enhance Cosmomycin production, a secondary metabolite, by mitigating negative feedback (Castillo et. al 2022).
St(r)epD (BBa_K4590005) shares a 99% identity with a gene encoding for a glutathione peroxidase, acting as an antioxidant to counteract the damage caused by free radicals in Streptomyces (Arteaga, 2015).
St(r)epE (BBa_K4590006) encodes a UvrA-like protein, involved in DNA repair in Streptomyces (Arteaga, 2015).
Both antioxidant action and DNA repair mechanisms are associated with self-resistance to damage caused by anthracyclines. Our intention is to boost Cosmomycin biosynthesis investigating the influence of all the 4 resistance genes on its production (Arteaga, 2015).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 199
Illegal NgoMIV site found at 394
Illegal NgoMIV site found at 402
Illegal NgoMIV site found at 431
Illegal NgoMIV site found at 865
Illegal NgoMIV site found at 1120
Illegal NgoMIV site found at 1270
Illegal AgeI site found at 1307 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1261
Illegal BsaI.rc site found at 3492
Illegal BsaI.rc site found at 4434
Illegal SapI site found at 2882
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