Coding
Str(e)pC

Part:BBa_K4590003

Designed by: Beatriz Lauriano de Oliveira   Group: iGEM23_USP-Brazil   (2023-10-02)


ABC Transporter Membrane Protein

St(r)epC (BBa_K4590003) is used in conjunction with St(r)epB (BBa_K4590002). It encodes an ABC Transporter Membrane Protein, which is a subunit of an ABC transporter. Membrane transporters are mechanisms of antibiotic resistance, as they decrease the concentration of these compounds inside the cell. By overexpressing the ABC transporter, we aim to increase the production of Cosmomycin, a secondary metabolite, through negative feedback (Arteaga et. al 2022).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 130
    Illegal NgoMIV site found at 280
    Illegal AgeI site found at 317
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 271


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Categories
Parameters
None