Coding
Str(e)pB

Part:BBa_K4590002

Designed by: Beatriz Lauriano de Oliveira   Group: iGEM23_USP-Brazil   (2023-10-02)


ABC Transporter ATP Binding Protein

St(r)epB (BBa_K4590002) is used in conjunction with St(r)epC (BBa_K45900003). It encodes an ABC Transporter ATP Binding Protein, which is a subunit of an ABC transporter. Membrane transporters are mechanisms of antibiotic resistance, as they decrease the cytoplasmic concentration of these compounds sending them to the extracellular medium. Intracellular accumulation of antibiotics can trigger negative feedback systems as mechanisms of defense to avoid self-intoxication. By overexpressing the ABC transporter, we aim to increase the export of Cosmomycin, a secondary metabolite, minimizing possible feedback mechanisms (Arteaga et. al 2022).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 199
    Illegal NgoMIV site found at 394
    Illegal NgoMIV site found at 402
    Illegal NgoMIV site found at 431
    Illegal NgoMIV site found at 865
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None