Part:BBa_K4588041
Contents
Rosmarinic acid synthase(Saccharomyces cerevisiae), RAS
This part encodes rosmarinic acid synthase from Plectranthus scutellarioides
Biology
The organism this gene is initially expressed in is P. scutellarioides.
The plant leaves of P.scutellarioides contain high levels of rosmarinic acid. Rosmarinic acid synthase catalyzes the coupling of 4-coumaroyl-CoA and 4-HPL by releasing coenzyme A, synthesizing rosmarinic acid [1].
Design
This BioBrick contains a GAL1 promoter (BBa_K2637059) that supports strong inducible expression in the presence of galactose. Following this promoter, the sequence contains a Kozak sequence (BBa_J63003) for optimal ribosome binding. The protein coding sequence, BBa_K4588030, was optimized for S. cerevisiae expression. The coding region also contains a 6x His tag (BBa_K1223006) for protein detection. The strong ADH1 terminator (BBa_K1486025) completely stops protein expression.
Usage
This enzyme is implemented in the synthesis pathway to produce rosmarinic acid in the S. cerevisiae culture. RAS converts caffeoyl CoA and salvianic acid A to produce rosmarinic acid by a condensation reaction[1].
References
1. Babaei, M., Borja Zamfir, G. M., Chen, X., Christensen, H. B., Kristensen, M., Nielsen, J., & Borodina, I. (2020). Metabolic Engineering of Saccharomyces cerevisiae for Rosmarinic Acid Production. ACS synthetic biology, 9(8), 1978–1988. https://doi.org/10.1021/acssynbio.0c00048
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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