Part:BBa_K4588037
Contents
Tyrosine ammonia lyase (S. cerevisiae), TAL
This part encodes Tyrosine ammonia lyase from Flavobacterium johnsoniaea K12
Biology
The organism this gene is initially expressed in is F. johnsoniaea.
In Flavobacterium johnsoniaea, TAL is responsible for converting L-tyrosine to p-coumaric acid. The enzyme forms a homo-tetramer when active as a cytosolic enzyme, mostly consisting of alpha helices. The enzyme FjTAL has been studied for the sustainable production of phenylpropanoids [1].
Design
This BioBrick contains a GAL1 promoter (BBa_K2637059) that supports strong inducible expression in the presence of galactose. Following this promoter, the sequence contains a Kozak sequence (BBa_J63003) for optimal ribosome binding. The protein coding sequence, BBa_K4588026, was optimized for S. cerevisiae expression. The coding region also contains a 6x His tag (BBa_K1223006) for protein detection. The strong ADH1 terminator (BBa_K1486025) efficiently stops protein expression.
Usage
This enzyme is implemented in the synthesis pathway to produce rosmarinic acid in the Saccharomyces cerevisiae culture. TAL converts L-tyrosine to p-coumaric acid with the removal of the amine group. [3].
References
1. SeyyedAmirreza Mousavi Majd. (2022). Flavobacterium johnsoniae Tyrosine Ammonia Lyase (FjTAL) in-silico Structure Prediction and Molecular Docking to L-Tyrosine, p-Coumaric Acid (pHCA) and Caffeic Acid. bioRxiv, 2022.02.09.479702. https://doi.org/10.1101/2022.02.09.479702
2. Babaei, M., Borja Zamfir, G. M., Chen, X., Christensen, H. B., Kristensen, M., Nielsen, J., & Borodina, I. (2020). Metabolic engineering of saccharomyces cerevisiae for rosmarinic acid production. ACS Synthetic Biology, 9(8), 1978–1988. https://doi.org/10.1021/acssynbio.0c0004
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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