Part:BBa_K4588030

Rosmarinic acid synthase(S. cerevisiae), RAS

This part encodes rosmarinic acid synthase from Plectranthus scutellarioides

Biology

The organism this gene is initially expressed in is P. scutellarioides

The plant leaves of P.scutellarioides contain high levels of rosmarinic acid. Rosmarinic acid synthase catalyzes the coupling of 4-coumaroyl-CoA and 4-HPL by releasing coenzyme A, synthesizing rosmarinic acid [1].

Usage

This enzyme is implemented in the synthesis pathway to produce rosmarinic acid in the S. cerevisiae culture. RAS converts caffeoyl CoA and salvianic acid A to produce rosmarinic acid by a condensation reaction[1]

References

1. Babaei, M., Borja Zamfir, G. M., Chen, X., Christensen, H. B., Kristensen, M., Nielsen, J., & Borodina, I. (2020). Metabolic Engineering of Saccharomyces cerevisiae for Rosmarinic Acid Production. ACS synthetic biology, 9(8), 1978–1988. https://doi.org/10.1021/acssynbio.0c00048

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]