Part:BBa_K4588028

4-hydroxy phenylacetate 3-monooxygenase reductase component C (S. cerevisiae), HpaC

This part encodes 4-hydroxy phenylacetate 3-monooxygenase reductase component C from Lysinibacillus capsici

Biology

The organism this gene is initially expressed in is L. capsici.

HpaC works with HpaB together to catalyze the oxidation of 4-hydroxyphenylacetate in the presence of NADH and FAD [1]. HpaC is a coupling factor that enhances NADH oxidation for HpaB [1].

Usage

This enzyme is implemented in the synthesis pathway to produce rosmarinic acid in the Saccharomyces cerevisiae culture. HpaC, in complex with HpaB, is part of the two-step process of converting p-coumaric acid into caffeic acid, which adds a hydroxyl group onto the phenyl ring [2].

References

1. Xun, L., & Sandvik, E. R. (2000). Characterization of 4-hydroxyphenylacetate 3-hydroxylase (HpaB) of Escherichia coli as a reduced flavin adenine dinucleotide-utilizing monooxygenase. Applied and environmental microbiology, 66(2), 481–486. https://doi.org/10.1128/AEM.66.2.481-486.2000

2. Babaei, M., Borja Zamfir, G. M., Chen, X., Christensen, H. B., Kristensen, M., Nielsen, J., & Borodina, I. (2020). Metabolic engineering of saccharomyces cerevisiae for rosmarinic acid production. ACS Synthetic Biology, 9(8), 1978–1988. https://doi.org/10.1021/acssynbio.0c0004

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]