Part:BBa_K4588024

4-hydroxy phenylacetate 3-monooxygenase reductase component C (E. coli), HpaC

This part encodes 4-hydroxy phenylacetate 3-monooxygenase reductase component C from Pasteurella multocida

Biology

The organism this gene is initially expressed in is Pasteurella multocida

HpaC is a coupling factor that enhances NADH oxidation for HpaB. This enzyme works with HpaB together to catalyze the oxidation of 4-hydroxyphenylacetate in the presence of NADH and FAD [1].

Usage

This enzyme is implemented in the synthesis pathway to produce salvianic acid A in an E. coli culture. This enzyme converts 4-hydroxyphenylpyruvate into 3,4-dihydroxy phenylpyruvate by adding a hydroxyl group to the benzene ring [2].

References

1. Xun, L., & Sandvik, E. R. (2000). Characterization of 4-hydroxyphenylacetate 3-hydroxylase (HpaB) of Escherichia coli as a reduced flavin adenine dinucleotide-utilizing monooxygenase. Applied and environmental microbiology, 66(2), 481–486. https://doi.org/10.1128/AEM.66.2.481-486.2000

2. Bloch, S. E., & Schmidt‐Dannert, C. (2014). Construction of a chimeric biosynthetic pathway for the de novo biosynthesis of rosmarinic acid in Escherichia coli. ChemBioChem, 15(16), 2393–2401. https://doi.org/10.1002/cbic.201402275

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]