Composite

Part:BBa_K4585016

Designed by: Siyao Ma   Group: iGEM23_CSU-CHINA   (2023-10-08)

pcDNA3.1(+)-GnRH-C linearized vector

We based on the sequence of the pcDNA3.1(+)-GnRH-C plasmid. Special primers were designed to cut the plasmid into a linear sequence by PCR. The two ends of this linear sequence can be complementary to the GnRH-C homologous recombination fragment to obtain the target product, the pcDNA3.1(+)-GnRH-C plasmid (BBa_K4585011).

1 Diagrams


Fig 1. The agarose gel electrophoresis of pcDNA3.1(+)-GnRH-C linearized vector

2 Caution

The two ends of the pcDNA3.1(+)-GnRH-C linearized vector need to be complementary to the two ends of the corresponding recombinant fragments to ensure the success of homologous recombination ligation.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 249
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
    Illegal XhoI site found at 901
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 249
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 249
    Illegal NgoMIV site found at 1341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882
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