Composite

Part:BBa_K4578006

Designed by: Kelly Yoon   Group: iGEM23_USAFA   (2023-10-05)


OspA expression block

Outer surface protein for B. burgdorferi, target of detection due to its high concentration within 72 hours of a bite. Used to modify E. coli to act as a model organism in Lyme disease research.

Optimizing this sequence for E. coli allows for the design of a model organism that satisfies BSL-1 laboratory requirements. This provides more opportunities to test novel Lyme disease detection systems in the future.

This expression block includes OspA and its respective T7 promoter, lac operon, and RBS.

OspA is expressed on the surface of B. burgdorferi and functions as a signal transducer or as a receptor. The OspA sequence we optimized is BBa_K3767004 from iGEM21_Queens_Canada.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None