Coding

Part:BBa_K4578001

Designed by: Kelly Yoon   Group: iGEM23_USAFA   (2023-10-05)


OspA

Outer surface protein for B. burgdorferi, target of detection due to its high concentration within 72 hours of a bite. Used to modify E. coli to act as a model organism in Lyme disease research.

Optimizing this sequence for E. coli allows for the design of a model organism that satisfies BSL-1 laboratory requirements. This provides more opportunities to test novel Lyme disease detection systems in the future.

OspA is expressed on the surface of B. burgdorferi and functions as a signal transducer or as a receptor. The OspA sequence we optimized is BBa_K3767004 from iGEM21_Queens_Canada.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 81
    Illegal EcoRI site found at 642
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 81
    Illegal EcoRI site found at 642
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 81
    Illegal EcoRI site found at 642
    Illegal BglII site found at 693
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 81
    Illegal EcoRI site found at 642
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 81
    Illegal EcoRI site found at 642
  • 1000
    COMPATIBLE WITH RFC[1000]


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