Part:BBa_K4575040
Cyanogen bromide cleavage site
A cyanogen bromide cleavage site is a specific peptide sequence recognized and cleaved by the chemical reagent cyanogen bromide (CNBr). CNBr is widely used in biochemistry to cleave peptide bonds at the carboxyl side of methionine residues in a protein or peptide. The cleavage occurs between the methionine and the following amino acid, generating two fragments. The reaction is highly selective for methionine and does not affect other amino acids, making it a valuable tool for protein sequencing and structural analysis.
In our project, we introduce a methionine residue at the C-terminus of our peptide to utilize CNBr cleavage for purification and analysis of the antimicrobial peptide.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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