Composite

Part:BBa_K4575015

Designed by: Sarah Hutzl, Sumohit Sharma   Group: iGEM23_Stuttgart   (2023-10-07)


Expression casette for Dispersin B expression in Pichia pastoris

DNA fragment which was designed for the expression of Dispersin B from Aggregatibaccter actinomycetemcomitans in the Pichia pastoris expression system. Dispersin B hydrolizes beta 1,6 glycosidic bonds of glucan polymers. Thus, it can attack the exopolysaccharides in a biofilm for example.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2405
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2239


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Categories
Parameters
None