Composite

Part:BBa_K4575014

Designed by: Sarah Hutzl, Sumohit Sharma   Group: iGEM23_Stuttgart   (2023-10-07)


Expression casette for Dextranase expression in Pichia pastoris

DNA fragment which was designed for the expression of Dextranase from Streptococcus mutans in the Pichia pastoris expression system. Dextranase hydrolizes alpha 1,6 and alpha 1,3 glycosidic bonds of glucan polymers. Thus, it can attack the exopolysaccharides in a biofilm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3132
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3644
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1832
    Illegal BsaI.rc site found at 1849
    Illegal BsaI.rc site found at 1942
    Illegal BsaI.rc site found at 2662


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