Coding

Part:BBa_K4575010

Designed by: Sumohit Sharma, Sarah Hutzl   Group: iGEM23_Stuttgart   (2023-10-06)


Dextranase from Streptococcus mutans for expression in Pichia pastoris

Dextranase from Streptococcus mutans optimized for expression in the Pichia pastoris expression system. Dextranase hydrolizes alpha 1,6 and alpha 1,3 glycosidic bonds of glucan polymers. Thus, it can attack the exopolysaccharides in a biofilm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1911
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 611
    Illegal BsaI.rc site found at 628
    Illegal BsaI.rc site found at 721
    Illegal BsaI.rc site found at 1441


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Parameters
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