Coding

Part:BBa_K4575009

Designed by: Sumohit Sharma, Sarah Hutzl   Group: iGEM23_Stuttgart   (2023-10-06)


Dispersin B from Aggregatibaccter actinomycetemcomitans for expression in Pichia pastoris

Dispersin B from Aggregatibaccter actinomycetemcomitans optimized for expression in the Pichia pastoris expression system. Dispersin B hydrolizes beta 1,6 glycosidic bonds of glucan polymers. Thus, it can attack the exopolysaccharides in a biofilm for example.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1018


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Categories
Parameters
None