Coding

Part:BBa_K4575008

Designed by: Sumohit Sharma, Sarah Hutzl   Group: iGEM23_Stuttgart   (2023-10-06)


Mutanase from Penicillium rubens for expression in Pichia pastoris

Mutanase from Penicillium rubens optimized for expression in the Pichia pastoris expression system. Mutanase hydrolizes alpha 1,3 glycosidic bonds of glucan polymers. Thus, it can attack the exopolysaccharides in a biofilm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1219
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3
    Illegal AgeI site found at 940
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None