Plasmid_Backbone

Part:BBa_K4516007

Designed by: Kong Yangyang   Group: iGEM22_Jiangsu_United   (2022-07-19)


pGL3pro-backbone

pGL3pro-backbone

contribution

pGL3pro-backbone is a high-copy-number luciferase reporter system carrier, which uses an SV40 promoter to regulate the expression of luciferase genes, and it can be used for rapid and quantitative assessment of factors that affect the expression of specific genes in mammalian cells. When expressed in the prokaryotic system, the Amp+ resistance can be used to screen the right colony. These new composite parts are important for both our project and future projects

Figure 1. The map of three plasmids pGL3pro-3IRE (B)..

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 58
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 2966
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 10
    Illegal BamHI site found at 320
    Illegal XhoI site found at 6
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 58
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 58
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 444
    Illegal NgoMIV site found at 2513
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1536
    Illegal SapI site found at 453


[edit]
Categories
Parameters
None