DNA

Part:BBa_K4503000

Designed by: Konstantinos Kariotis   Group: iGEM22_Anatolia_College_HS   (2022-09-28)


Coding sequence of NirS gene from Psedomonas azotoformans with NdeI and HindII restriction sites for

Coding sequence of NirS gene from Pseudomonas azotoformans. It codes for nitrate reductase. It contains a NdeI restriction site at the 5' and and a HindIII restriction site at the 3' end. For use for protein expression. No illegal restriction sites present.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 58
    Illegal NgoMIV site found at 124
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 606
    Illegal NgoMIV site found at 1447
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None