Composite

Part:BBa_K4501021

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-22)


Cytosolic delivery helper

This composite part contains the expression cassette for the Connexin43 with the mutation S368A, that enables a constitutive active conformation of the channel. It's main function is to serve as open channels for the RNAs to enter into the exosomes on the Multivesicular bodies.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1800
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3102
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1440
    Illegal SapI.rc site found at 1076


[edit]
Categories
Parameters
None