Composite
Part:BBa_K4501021
Designed by: Pau Marín Escudero Group: iGEM22_Barcelona_UB (2022-09-22)
Cytosolic delivery helper
This composite part contains the expression cassette for the Connexin43 with the mutation S368A, that enables a constitutive active conformation of the channel. It's main function is to serve as open channels for the RNAs to enter into the exosomes on the Multivesicular bodies.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1800
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3102
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1440
Illegal SapI.rc site found at 1076
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Categories
Parameters
None |