Composite

Part:BBa_K4501012

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-22)


Exosome booster 2

This composite part codes for an expression cassette containing the nSMase 2 gene, related with boosting of exosome biogenesis. The construction also includes a hygromycin resistance gene on a bycistron to facilitate the selection of cells that become transfected.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1130
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2267
    Illegal NgoMIV site found at 4231
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 936
    Illegal BsaI.rc site found at 1352
    Illegal BsaI.rc site found at 2258


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Parameters
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