Cell

Part:BBa_K4501010

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-09)


Connexin43 S368A mut

Connexin 43, or hGJA1[NM_000165.5], is a member of the connexin gene family, encoding a component of gap junctions. They provide a route for the diffusion of low molecular weight compounds from cell to cell. As for exosomes, this protein has recently been reported to be enriched in exosomes and to enhance cytosolic delivery of exosomes to acceptor cells (Soares AR et al., 2015). Its constitutive active mutant, Cx S368A, has been reported to be more efficient as cytosolic delivery helper (Kojima R et al., 2018).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1026
    Illegal PstI site found at 238
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1026
    Illegal PstI site found at 238
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1026
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1026
    Illegal PstI site found at 238
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1026
    Illegal PstI site found at 238
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 782
    Illegal SapI.rc site found at 418


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Parameters
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