Cell

Part:BBa_K4501009

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-09)


CD19-L

This part encodes for a ligand of CD19, a surface marker of B cells. It speciffically encodes for the active extracellular domain of the ligand, with no transmembrane nor cytosolic domain. Structure was obtained by homology approximation and then cytosolic and transmembrane domains were removed for it to be attached as a fusion protein.

Docksimple.jpg Figure 1. Image of the surface interaction of CD19-L (blue) with CD19 (red).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 97
    Illegal NgoMIV site found at 340
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42


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Parameters
None