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Part:BBa_K4501003

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-08)


AAVS1 5

Encodes for the 5' homology arm of the AAVS1 region of the human genome. This region is proven to be a safe harbor for integration of transgenes, allowing targeted integration and stable expression. Specifically, this sequence was designed by Seunghyeon Shin et al. using the genome of the HEK293 cell line, so it may only be efficient on that cell line and derived ones.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 136
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 669
    Illegal PstI site found at 136
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 313
    Illegal BamHI site found at 787
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 136
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 136
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 673


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Categories
Parameters
None