RNA

Part:BBa_K4501002

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-08)


anti-myc shRNA (1741-1761) in-loop C/D-box

Encodes for an shRNA against the 1741-1761 positions of the mRNA principal transcript of human MYC (HGNC:7553). It is composed of a sense and an anti-sense sequence separated by a loop domain. To optimize and stabilize the transcript, the C/D-box sequence is allocated at the position of the loop, substituting the loop.

BBa K4501002.jpeg Figure 1. Structure and stability of the shRNA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None