Coding

Part:BBa_K4497007

Designed by: Till Gundlach   Group: iGEM22_Munich   (2022-10-09)


Tetracycline-Induced Transactivator tTA

The Tetracycline-Induced Transactivarot tTA binds to the DNA sequence of the Tet Response Element TRE, inducing protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Kafri, T., van Praag, H., Gage, F. H., & Verma, I. M. (2000). Lentiviral vectors: Regulated gene expression. Molecular Therapy, 1(6), 516–521. https://doi.org/10.1006/mthe.2000.0083

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