Coding

Part:BBa_K4497000

Designed by: Till Gundlach   Group: iGEM22_Munich   (2022-10-09)


pH-sensitive protein linker (EAAAK)5

This linker sequence of 25 amino acids (EAAAK)5 has pH-sensitive auto-catalytic according to Wu et al.

Reference: Wu, Y. J., Fan, C. Y., & Li, Y. K. (2009). Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide. Journal of Chromatography B, 877(31), 4015–4021. https://doi.org/10.1016/J.JCHROMB.2009.10.009

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None