Primer

Part:BBa_K4496012

Designed by: Larissa de Souza Kawanisi, Awana da Silva Lima, Paula Macedo Cunha, Gabriel Kimijima Itaziki   Group: iGEM22_USP-EEL-Brazil   (2022-09-20)


Primer to amplify psB1C3 (FWD)

This is the foward primer designed to amplify the psB1C3 plasmid for 3A assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None