Device

Part:BBa_K4491145

Designed by: Kazimir Uzwyshyn Jones   Group: iGEM22_Cambridge   (2022-10-12)


Antithetic integral controller for positive perturbation (tetR , DSS)

Transcriptional unit implemented in the downstream site of the level 2 pJUMP47-2A. The transcriptional unit was assembled through a linear golden gate reaction. This was implemented after creation of a level 2 circuit. Addition of Atc (inhibitor) to tetR repressor allows expression at module D of X. This therefore acts as a positive perturbation to for the antithetic integral controller.

https://2022.igem.wiki/cambridge/parts

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 790
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None