Device
Part:BBa_K4491145
Designed by: Kazimir Uzwyshyn Jones Group: iGEM22_Cambridge (2022-10-12)
Antithetic integral controller for positive perturbation (tetR , DSS)
Transcriptional unit implemented in the downstream site of the level 2 pJUMP47-2A. The transcriptional unit was assembled through a linear golden gate reaction. This was implemented after creation of a level 2 circuit. Addition of Atc (inhibitor) to tetR repressor allows expression at module D of X. This therefore acts as a positive perturbation to for the antithetic integral controller.
https://2022.igem.wiki/cambridge/parts
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 790
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |