Device

Part:BBa_K4491115

Designed by: Yuen Shan Ho   Group: iGEM22_Cambridge   (2022-10-12)


MegaT_RFP

The design of MegaT is to design a part that contains RBS, mVenus and Terminator to allow modular JUMP assembly. In this case, to make our system modular, we have added SapI restriction enzyme cut sites on either end of the drop out casette of BBa_J04450 so that users can easily replace the dropout casette with the protein of their choice

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 792
    Illegal AgeI site found at 904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1075
    Illegal SapI.rc site found at 5


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Categories
Parameters
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