Coding
Part:BBa_K4488027
Designed by: Jessica, Sylvie, Kawana Group: iGEM22_Sydney_Australia (2022-10-11)
Neae-intimin Construct Displaying anti-sfGFP Nanobody H
Surface display of anti-sfGFP nanobody H using the bacterial protein Neae-intimin. The nanobody is a modification of PDB: 3OGO-H. There is a linker between the Neae-intimin gene and the nanobody sequence. It has been optimised to express in pUS250 TOP10 E.coli.
Usage and Biology
Our project demonstrated that anti-sfGFP nanobody H binds to sfGFP through a GFP bindings assay. Therefore, nanobody H can be effectively surface displayed using Neae-intimin which provides preliminary evidence that other types of nanobodies can be displayed and later selected using the same construct.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |