Coding
fuGFP-CBDc

Part:BBa_K4488010

Designed by: Donovan Wu, Jackie Yau, Oliver Nicholls, Jasmin Li   Group: iGEM22_Sydney_Australia   (2022-09-30)


Fusion of free-use GFP with CBDcenA (cellulose-binding domain) at the C-terminal end

The construct can be cloned into an expression vector such as pET28c in E.coli to produce a fusion protein of fuGFP with CBDcenA. The fuGFP sequence is towards the N terminus of the protein with CBDcenA (BBa_K1321339) downstream followed by a stop codon. Recognition sites for BamHI and BsaI are present before the RBS allowing golden gate cloning. XhoI and BsaI are also present downstream of the stop codon. Notably, the assembled protein is insoluble. See BBa_K4488014 for improved construct with higher fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 163
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None