Coding
fuGFP-CBDc
Part:BBa_K4488007
Designed by: Donovan Wu, Jackie Yau, Oliver Nicholls, Jasmin Li Group: iGEM22_Sydney_Australia (2022-09-30)
Fusion of free-use GFP with CBDclos (cellulose-binding domain) at the C-terminal end
The construct can be cloned into an expression vector such as pET28c in E.coli to produce a fusion protein of fuGFP with CBDclos. The fuGFP sequence is towards the N terminus of the protein with CBDclos (BBa_K1321341) downstream followed by a stop codon. Recognition sites for BamHI and BsaI are present before the RBS allowing golden gate cloning. XhoI and BsaI are also present downstream of the stop codon. Notably, the assembled protein is insoluble. See BBa_K4488011 for improved construct with higher fluorescence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 163
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |